BMG Labtech

PHERAstar FSX

The Ultimate

Multi-mode Microplate Reader

for High-throughput Screening

Video

Overview

The new gold standard for High-throughput Screening (HTS)

The PHERAstar^® FSX is the ultimate multi-mode microplate reader for high-throughput screening, combining extremely high sensitivity with very fast read times. Thanks to the unique Optic Module system, Simultaneous Dual Emission, UV/Vis spectrometer, and dedicated AlphaScreen^® and TRF lasers, whatever your application, the PHERAstar^® FSX will perform it with ease and speed, even in 3456-well microplates.

Features & Benefits

The only microplate reader with 5 assay-dedicated detectors
The most sensitive microplate reader in Fluorescence Intensity and Polarization
Fastest read times with Simultaneous Dual Emission, including for Alpha Technology
Dedicated laser for AlphaScreen^®/AlphaLISA^®/AlphaPlex™
Dedicated new generation laser for TRF and TR-FRET
9 decades dynamic range in Luminescence
Ultra fast spectral scanning in Absorbance (from 220 to 1000 nm in less than 1 second per well)
Well scanning with up to 900 data points per well for non-homogeneous samples (e.g. cell-based assays)
Automated focus (focal height) adjustment for both top and bottom readings
High-precision injectors capable of simultaneous reagent injection and detection
Measurements in up to 3456-well microplate format
Linear, orbital and double-orbital shaking with user-definable time and speed
Incubation up to 45°C with 0.1°C resolution
Multi-user Reader Control Software with unlimited license
Multi-user MARS Data Analysis Software with unlimited license
FDA 21 CFR Part 11 compliance in software
Data export to Excel, Absolute Database or ASCII format
Easy integration with LIMS systems
Easy integration with robotic systems for heavy workload and lab automation
Compatibility with Evaluation Plate for GLP compliance and LVis Plate for low-volume (down to 2µl) samples

Technical Specifications

Detection Modes	UV/Vis Absorbance Fluorescence Intensity - incl. FRET Luminescence (flash and glow) - incl. BRET Fluorescence Polarization Time-resolved Fluorescence (TRF) Time-resolved FRET (TR-FRET) AlphaScreen^®/AlphaLISA^®/AlphaPlex™
Measurement Modes	Top and bottom reading Endpoint and kinetic measurements Sequential multi-excitation measurements Sequential multi-emission measurements Simultaneous dual emission measurements Spectral scanning (ABS) Real-time ratiometric measurements Well scanning
Microplate Formats	6 to 3456 well plates, user-definable LVis Plate with 16 low volume microspots (2 μL)
Microplate Carrier	Robot compatible
Light Sources	High energy Xenon flash lamp Dedicated laser for AlphaScreen^®/AlphaLISA^®/AlphaPlex™ Dedicated laser for TRF and TR-FRET
Detectors	Two matched pairs of photomultiplier tubes (PMTs), dedicated to different detection modes CCD spectrometer (ABS)
Wavelength Selection	Optic Modules Up to 6 application-specific modules (includes all filters, dichroics, etc., for an application) for top and bottom reading UV/Vis Absorbance Spectrometer Full spectra or 8 discrete wavelengths in ‹1 sec/well
Optical Path Guides	Top and bottom: Free-air optical light path guided by motor-driven mirrors and dichroics
Z-Adjustment	Automatic focal height adjustment (0.1 mm resolution)
Spectral Range	Filters 230 - 750 nm or 230 - 900 nm (optional) for FI, FP 230 - 750 nm for LUM 230 - 900 nm for TRF Spectrometer 220 - 1000 nm for ABS Wavelength precision: ≤0.5 nm
Sensitivity	FI (top) ‹0.15 pM FITC (384sv black, 20 μL) ‹0.5 pM FITC (1536 black, 8 μL) FI (bottom) ‹1.0 pM FITC (384g black, 50 μL) FP ‹0.5 mP SD at 1 nM FITC (384sv black, 20 μL) ‹1.5 mP SD at 1 nM FITC (1536 black, 8 μL) HTRF^® (black and white microplates) Reader Control Kit (Eu) after 18h (384sv, 20 μL) Delta F ›1100% (High Calibrator) Delta F ›25% (Low Calibrator) TRF with Laser ‹5 fM europium (384, 80 μL) ‹15 fM europium (1536, 8 μL) LUM ‹0.4 pM (‹8 amol/well ATP, 384sv white, 20 μL) Dynamic range: 9 decades AlphaScreen^® with Laser ‹5 pM (‹100 amol/well P-Tyr-100, 384sv white, 20 μL) ABS with Spectrometer Selectable spectral resolution: 1, 2, 5 and 10 nm OD range: 0 to 4 OD Photometric resolution: 0.001 OD Accuracy: ‹1% at 2 OD Precision: ‹0.5% at 1 OD and ‹0.8% at 2 OD Linearity: ≤0.8% at 2.0 OD
Read Times	Flying mode (1 flash): 14 sec (384), 27 sec (1536) 10 flashes: 38 sec (384), 1 min 52 sec (1536) 50 flashes: 1 min 29 sec (384), 5 min 18 sec (1536)
Shaking	Linear, orbital and double-orbital with user-definable time and speed
Incubation	+5°C above ambient up to 45°C The upper heating plate of the incubation chamber operates at 0.5 °C more than the lower plate. This prevents condensation build-up on the lid or sealer.
Software	Multi-user Reader Control Software & MARS Data Analysis Software FDA 21 CFR Part 11 compliant
Dimensions & Weight	Width: 45 cm, Depth: 51 cm, Height: 47 cm; Weight: 49 kg
Optional Accessories	Stacker IIIPlate handler for up to 50 microplates - continuous loading feature Reagent Dispenser ModuleUp to 2 built-in reagent injectors Injection at measurement position (6 to 384-well) Individual injection volumes for each well: 3 to 500 μL (optionally up to 2 mL) Variable injection speed up to 420 μL/sec Up to 4 injection events per well Reagent back flushing LVis PlateMicroplate designed to measure 16 low volume (2 μL) samples and standard cuvettes. Incorporates NIST-traceable filters and holmium oxide standards for instrument performance test. Sensitivity: 2 ng/μL dsDNA Evaluation PlatePlate designed to validate the optical functionality of microplate reader for quality control and/or qualification (IQ/OQ) purposes in environments following GLP compliance. THERMOstarMicroplate incubator and shaker Integrated Barcode ReaderUp to 3 integrated barcode readers

ABS: Absorbance / FI: Fluorescence Intensity / LUM: Luminescence / FP: Fluorescence Polarization / TRF: Time-resolved Fluorescence / TR-FRET: Time-resolved FRET FI (incl. FRET), FP and LUM are the standard detection modes. Other detection modes are optional.

All or some of the specifications and features noted on this page may be optional. All necessary details in this regard may not be specified on this page. Please contact us for full details. / Product design, appeareance, functionality, features and specifications are subject to change without notice.